Especies crípticas Lutzomyia longipalpis (Diptera: Phlebotominae) y sus implicaciones en la en la transmisión de leishmaniasis en Panamá.

Anayansi Valderrama Cumbrera, Mileyka Santos, Angélica Castro, José Dilermando Andrade Filho

Resumen


[Cryptic Species Lutzomyia longipalpis (Diptera: Phlebotominae) and its Implications in the Transmission of Leishmaniasis in Panama]

RESUMEN

Lutzomyialongipalpises el principal vector de una importante enfermedad desatendida en América. La diversidad genética de este vector se estimó en la población colectada en dos áreas geográficas separadas por hasta 37 km. Analizamos la secuencia CB3-PDR / N1N-PDR de 22 individuos obteniendo un parámetro de: h = 0.43 y π = 0.0017 (Bo-na), h = 0.89, π = 0.004 (El Limón) con una diferenciación genética de kst = 0.03; p> 0.05 entre ellos. Ocho haplotipos fueron detectados, de los cuales fue compartido. Se detectó una diferenciación significativa entre las poblaciones Panamá-Colombia (kst = 0.98), Panamá-Costa Rica (kst = 0.98) y Panamá-Brasil (kst = 0.72) bajo el modelo de aislamiento. Las inferencias genéticas de esta población pueden complementar la información de la capacidad de dispersión y brindar pistas importantes para comprender la ecología de Lulongiplapisen Panamá.

ABSTRACT

Lutzomyia longipalpisis the main vector-borne of important neglected disease in America. The genetic diversity of this vector was estimated in population collected from two geographic area separated by up to 37km. We analyzed the sequence CB3-PDR/N1N-PDR of 22 individuals and the resulted of parameter was: h = 0.43 and π = 0.0017 (Bona), h = 0.89, π = 0.004 (El Limón) and genetic differentiation was kst = 0.03; p > 0.05 among them. Eight haplotypes were detected, and one was shared. Significant differentiation was detected among populations Panama-Colombia (kst = 0.98), Panama-Costa Rica (kst = 0.98) and Panama-Brazil (kst = 0.72) and these were genetically isolated by distance. The existence of shared haplotypes among the populations suggests a gene flow despite the distance and geographic separation. Nevertheless, showed isolation it contrasted with individuals very distant.The genetic inferences of this population can supplement the information of dispersal capacity and provided important clues to understand the ecology of sandflies.


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Referencias


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